The first 40 tonnes of parts were loaded in Yogyakarta, Indonesia, while the rest was loaded in Hanoi, Vietnam.
The Open Skies Agreement is between the Ukraine and US, where it promotes an increase in travel, trade, productivity, and economic growth between the two nations.
'We have seen an increase in demand for automotive cargo, and a double pick-up is not usual in the day-to-day operations of the AN-124-100,' said Antonov Airlines commercial executive Iryna Kyianytsia.
'There were additional challenges ensuring compliance with Covid-19 restrictions in different nations, but thanks to well-organised work and communication with The Flight Lab Aviation Consulting LLC, which chartered the flights, the cargo was safely and successfully transported in full and on time,' said Ms Kyianytsia.
It took four days to complete the route. The crew had stops in Japan and Alaska to accommodate crew rests and aircraft refuelling.
'These flights were not your run-of-the-mill charters. Due to the nature of the cargo and the double pick-up in two different countries, the planning had to be precise and executed perfectly,' said Flight Lab Aviation Consulting founder Adam Green.
'All the cargo was Just-In-Time, to support a large automotive manufacturer; with the help of the consignee V. Alexander in the USA, each flight went off without a hitch,' said Mr Green.