It also reserved four other registration numbers, although aircraft have yet to allocated to these numbers, all prime numbers.
Up until now, the e-commerce giant has run its air cargo business through lease deals with ATSG, Atlas Air and Sun Country.
According to aircraft tracking websites, the plane - previously operated by WestJet - was put into storage in March as it awaited conversion.
It was then temporarily moved to Lake City Municipal before being flown to Tel Aviv in September, suggesting conversion work will be carried out by IAI.
Website PAXEX.AERO said that a second WestJet B767 has been moved to Lake City, while it has two more in storage in Calgary.
The B767-300 makes up most of the Amazon Air fleet of more than 50 aircraft, which also includes B737s.
Elsewhere, the F also granted the company with an exemption to carry out tests of its MK27 drone through its Part 135 AOC.
Amazon is currently trying to obtain a type certificate for the drone.